• DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution 

      Wang, Xumei; Gusarova, Galina; Ruhsam, Markus; de Vere, Natasha; Metherell, Chris; Hollingsworth, Peter M.; Twyford, Alex D. (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-04-24)
      DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically ...
    • The Future of DNA Barcoding: Reflections from Early Career Researchers 

      Grant, Danielle M.; Brodnicke, Ole Bjørn; Evankow, Ann; Ferreira, André O.; Fontes, João T.; Hansen, Aslak Kappel; Jensen, Mads Reinholdt; Kalaycı, Tuğba Ergül; Leeper, Alexandra Eve Joyce; Patil, Shalaka Kiran; Prati, Sebastian; Reunamo, Anna; Roberts, Aradhana J; Shigdel, Rajesh; Tyukosova, Valentina; Bendiksby, Mika; Blaalid, Rakel; Costa, Filipe O.; Hollingsworth, Peter M.; Stur, Elisabeth; Ekrem, Torbjørn (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-09)
      Over the last two decades, the use of DNA barcodes has transformed our ability to identify and assess life on our planet. Both strengths and weaknesses of the method have been exemplified through thousands of peer-reviewed scientific articles. Given the novel sequencing approaches, currently capable of generating millions of reads at low cost, we reflect on the questions: What will the future bring ...
    • The treasure vault can be opened: large-scale genome skimming works well using herbarium and silica gel dried material 

      Alsos, Inger Greve; Lavergne, Sebastien; Merkel, Marie Kristine Føreid; Boleda, Marti; Lammers, Youri; Alberti, Adriana; Pouchon, Charles; Denoeud, France; Pitelkova, Iva; Pușcaș, Mihai; Roquet, Cristina; Hurdu, Bogdan-Iuliu; Thuiller, Wilfried; Zimmermann, Niklaus E.; Hollingsworth, Peter M.; Coissac, Eric (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-04-01)
      Genome skimming has the potential for generating large data sets for DNA barcoding and wider biodiversity genomic studies, particularly via the assembly and annotation of full chloroplast (cpDNA) and nuclear ribosomal DNA (nrDNA) sequences. We compare the success of genome skims of 2051 herbarium specimens from Norway/Polar regions with 4604 freshly collected, silica gel dried specimens mainly from ...